【作者】张立

【导师】白虹

【作者基本信息】天津医科大学，免疫学，2007年，硕士

【摘要】 目的探讨正常妊娠妇女在妊娠过程中IL-1β、IL-6和TNF-α分泌及表达随人绒毛膜促性腺激素hCG水平的变化规律。方法采用放射免疫测定法检测正常妊娠妇女在妊娠早、中、晚期血清hCG、TNF-α、IL-1β和IL-6水平；采用逆转录-聚合酶链反应检测妊娠不同时期PBMC IL-1β、IL-6 mRNA表达，并与未孕妇女(对照组)进行对照研究。结果1．妊娠早期血清hCG浓度迅速升高，妊娠中晚期明显降低，但与正常对照组比较仍有显著性差异(p 0.01)，妊娠晚期降至高峰的20％左右。2．IL-1β血清水平在妊娠早期达到较高水平，中期有所下降，晚期又有所升高。妊娠不同时期血清IL-1β浓度变化随hCG浓度变化而改变，提示妊娠期间正常分泌的hCG可能在细胞因子的产生中发挥作用。妊娠不同时期，PBMC中均表达较高水平IL-1βmRNA，尤以晚期IL-1βmRNA表达升高更为明显，与血清IL-1β浓度的变化一致。3．与对照组比较，正常妊娠TNF-α血清水平在妊娠早、中、晚期均有所下降，但差异无显著性(p＞0.05)。4．正常妊娠早期血清IL-6水平及PBMC IL-6 mRNA的表达均随血清hCG...更多浓度增高而增加，至妊娠中期血清IL-6水平及PBMC IL-6 mRNA的表达持续增高，妊娠晚期达高峰。5．妊娠早期血清IL-1β／IL-6浓度的比值与对照组比较无显著性差异(p＞0.05)，而妊娠中期和晚期差异有显著性(p 0.01)。血清TNF-α/IL-6的浓度比值在妊娠早期、中期与对照组相比无显著差异(p＞0.05)，晚期与对照组相比差异有显著性(p 0.01)。血清TNF-α／IL-6和IL-1β/IL-6比值的变化，在一定程度上体现了妊娠期间宿主的免疫反应由Th1向Th2型转变的趋势。结论1．妊娠期间细胞因子IL-1β和IL-6血清水平及基因表达增高；2．妊娠期间正常分泌的hCG可能在细胞因子的产生中发挥作用；3．妊娠期间宿主的免疫反应可能发生了Th1向Th2型的转变。  还原

【Abstract】 Objective: To investigate the change of the secretion and expression ofIL-1β, IL-6 and TNF-αfollowing the level of hCG in normal pregnancy.Method: To test the level of hCG, TNF-a, IL-6 and IL-1βin the serum fromnormal pregnant women of the early, mid and late trimester of pregnancy by RIA. Totest the expression of IL-1β, IL-6 mRNA in PBMC in the different phasepregnancy by RT-PCR, while they contrasted to normal control group.Result:1. The level of serum hCG in the early pregnancy significantly increased, in themid and late trimester of pregnancy significantly decreased, but there was significantlydifference in the secretion of hCG between control group and pregnancy group(P＜0.01). The level of serum hCG in the late trimester pregnancy descended to the20％of the peak.2. A high level of IL- 1βin the serum is detected in the trimester of pregnancy, itdecreases in the mid pregnancy, increases again in the late trimester of pregnancy.The level of serum IL-1βchanges following the variation o...更多f hCG concentration, itreveals that the normocrinic hCG may play a role in the production of cytokine ingestational period. In the different periods of trimester of pregnancy, the expression ofIL-1βmRNA lasts a high level in PBMC, especially in the late trimester ofpregnancy, its variation according with the changing level of IL-1βin serum.3. Compare to the control group, the level of TNF-αin serum was drecreased inthe different periods of normal pregnancy, but it was not different significantlycompare to the normal group.4. The level of IL-6 in serum and the expression of PBMC IL-6 mRNA wereincreased following the increasing level of hCG in serum, they kept increasingduring the mid trimester of pregnancy and arrived at the peak in the late trimester of pregnancy.5. Compare to the control group, the ratio of IL-1β/IL-6 is not significantlydifferent in the early trimester of pregnancy (p＞0.05), but it has a significantlydifferent in the mid and late trimester of pregnancy (p＜0.01). The ratio of TNF-α/IL-6is not significantly different in the early and mid trimester of pregnancy (p＞0.05), butit has a significantly different in the late trimester of pregnancy (p＜0.01). To someextent, the change of the ratio of IL-1β/IL-6 and TNF-α/IL-6 demonstrate thetendency of transformation in host immune response from Thl to Th2 in thegestational period.Conclusion1. The level of IL-1βand IL-6 in serum and gene expression during thegestational period increased.2. The normocrinic hCG may play a role in the production of cytokine during thegestational period.3. The host immune response may transform from Th1 to Th2 during thegestational period.  还原

【关键词】 人绒毛膜促性腺激素; 白细胞介素-1β; 白细胞介素-6; 肿瘤坏死因子-α; 妊娠
【Key words】 human chorionic gonadotropin (hCG); Interleukin-1 beta (IL-1β); Interleukin-6 (IL-6); Tumor necrosis factor Alpha (TNF-α); Pregnancy

【网络出版投稿人】天津医科大学【网络出版投稿时间】2007-10-23 【网络出版年期】2007/06

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